Curdlan-dependent CTL crosspriming protects mice from tumor challenge. C57Bl/6 mice were immunized in both hind footpads with OVA protein alone (none) or together with curdlan or polyI:C as indicated. Melanoma cells were injected intravenously 5 to 7 days after immunization and tumor growth was analyzed 18 to 22 days thereafter. (A) Data represent tumor counts in each mouse from 2 pooled experiment (n = 10-11 mice/group). Lungs of 2 representative mice from each group are shown below the graph. (B) Frequency of SIINFEKL-H2K^b tetramer⁺ CD8⁺ cells in spleens from mice of 1 of 2 experiments shown in panel A. (C) Splenocytes from individual mice in 1 of 2 experiments shown in panel A were restimulated in vitro with SIINFEKL peptide. Data show CTL activity after restimulation as in Figure 6D.