Curdlan-stimulated DCs produce IL-12 p70 in an IFN-γ–dependent manner. (A) C57BL/6 BMDCs and OT-I were cocultured in the presence or absence of SIINFEKL peptide, curdlan (left) or CpG (right) as indicated. (B) As in panel A, but with the addition of anti–IFN-γ blocking antibody or rat IgG1 isotype-matched control antibody. (C) C57BL/6 BMDCs were stimulated with curdlan or CpG and recombinant IFN-γ as indicated. Data in all panels are IL-12 p70 levels in the supernatant determined after 24 hours (mean plus or minus SD of triplicate wells).