Curdlan-induced CTL priming by DC is dependent on the dectin-1 pathway. (A) Cocultures of OT-I T cells and either C57BL/6 (WT) or dectin-1–deficient BMDCs in the presence of 25 pM SIINFEKL peptide and no stimulus, curdlan, or CpG. Cultures were restimulated on day 3 with SIINFEKL-pulsed target T cells and IFN-γ levels in the supernatant were determined by ELISA. (B) As in panel A, but using Syk-deficient or MyD88 × TRIF doubly deficient BMDCs and 30 pM SIINFEKL peptide. Data in panels A and B are mean plus SD of duplicate wells.