Curdlan-stimulated DCs prime CTLs in vitro. (A) CFSE-labeled OT-I T cells were cultured together with C57Bl/6 BMDCs in the presence of the indicated concentrations of SIINFEKL peptide and either curdlan, CpG, or no innate stimulus (unstim). Graphs show CFSE dilution versus intracellular IFN-γ on day 3 of culture. Numbers indicate percent of IFN-γ⁺ cells. (B) The extent of proliferation of the T cells from experiment shown in panel A calculated as division index. (C) CD8⁺ T-cell counts per well from the experiment shown in panel A. (D) IFN-γ⁺CD8⁺ T-cell counts per well (top) and percentage of IFN-γ⁺ cells (bottom) from the experiment shown in panel A. (E) Samples from the experiment shown in panel A were restimulated on day 3 with SIINFEKL-pulsed targets for 8 hours and IFN-γ levels in the supernatant were determined by ELISA. Data in panels B through E are mean plus or minus standard deviation (SD) of duplicate wells.