American Society of Hematology

Figure 2.

Syngeneic MSCs support long-term engraftment. (A) T-cell–depleted BM from BALB/b mice (10 × 106) was transplanted alone or together with B6 MSCs (0.25 × 106) into 5 Gy-irradiated B6 recipients. (B) BALB/c mice were irradiated with 6 Gy and engrafted with T-cell–depleted BM (10 × 106) from B6 mice with or without BALB/c MSCs (0.25 × 106). MSCs were infused 5 times at days 0, 3, 7, 10, and 14 after BM transplantation. Peripheral blood was harvested and analyzed by flow cytometry to determine chimerism 1, 2, and 4 months after BM transplantation by the expression of Ly5.1 (A) or MHC class I (B). Data presented are pooled from 2 experiments. Horizontal bar indicates the average percentage of positive staining of donor cells for each group. †Number of engrafted mice/number of analyzed mice. *P < .05.

Syngeneic MSCs support long-term engraftment. (A) T-cell–depleted BM from BALB/b mice (10 × 10⁶) was transplanted alone or together with B6 MSCs (0.25 × 10⁶) into 5 Gy-irradiated B6 recipients. (B) BALB/c mice were irradiated with 6 Gy and engrafted with T-cell–depleted BM (10 × 10⁶) from B6 mice with or without BALB/c MSCs (0.25 × 10⁶). MSCs were infused 5 times at days 0, 3, 7, 10, and 14 after BM transplantation. Peripheral blood was harvested and analyzed by flow cytometry to determine chimerism 1, 2, and 4 months after BM transplantation by the expression of Ly5.1 (A) or MHC class I (B). Data presented are pooled from 2 experiments. Horizontal bar indicates the average percentage of positive staining of donor cells for each group. †Number of engrafted mice/number of analyzed mice. *P < .05.

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