MDCs matured with R-848 demonstrate enhanced HIV-1 transmission capacity, which is not due to increased viral capture. (A) MDCs were cultured in medium only, or with R-848 or IFNγ plus MF for 24 hours, followed by HIV-1 incubation and extensive washing to remove all factors. DCs were subsequently cocultured with LuSIV cells to allow HIV-1 transmission. Luciferase was measured after 24 hours. *P < .01, compared with medium and IFNγ/MF maturation. RLU, relative light units. (B) Differently matured MDCs as indicated on the x-axis were incubated for 4 hours with HIV-1, followed by extensive washing to remove unbound virus. Viral capture was subsequently determined by lysis of the cells and CA-p24 ELISA. Error bars represent standard deviations. NS, not significant.