Qualitative reverse transcription–polymerase chain reaction (qRT-PCR) analysis of C/EBPβ mRNA levels and protein expression in NPM-ALK and NPM-ALK-ATP-Abl–transformed Ba/F3 cells. (A) Western blot analysis of transformed Ba/F3 cells. Each lane contains 30 μg protein extract. The ALCL cell line Karpas 299 is used as control. Stat3 phosphorylate is used as control for the kinase activity of NPM-ALK. The NPM-ALK-ATP-Abl construct produces a positive protein band of 85 kDa. Tubulin is used as loading control. (B) qRT-PCR analysis of C/EBPβ was performed relative to the TBP housekeeping gene. Data were analyzed according to the ΔC_T method. Results are depicted as mRNA fold induction compared with Ba/F3 parental cells. Error bars indicate SD.