ALK, Stat3-phosphorylated and C/EBPβ expression in NPM-ALK and NPM-ALK-ATP-Abl–transformed Ba/F3 and 3D cells. (A) Western blot analysis of NPM-ALK–transformed Ba/F3 and 32D cell lines. Each lane contains 30 μg protein extract. The ALCL cell line Karpas 299 is used as control. The NPM-ALK construct produces a band of 75 kDa. Tubulin is used as loading control. (B) Western blot analysis of Ba/F3 NPM-ALK-ATP-Abl–transformed cell line untreated or treated with 5 μM imatinib for 12 hours. The NPM-ALK-ATP-Abl construct produces a positive protein band of 85 kDa, 5 kDa larger than the protein band detected in the SUDHL-1 cell line used as control. Tubulin is used as loading control.