Western blot analyses for C/EBPβ and ALK in lymphoid cell lines and primary lymphoma cases. (A) Subcellular fractionation of the SUDHL-1 cell line. Tubulin is used as control for loading and for the cytoplasmic fraction. (B) KiJk, Karpas 299, SUDHL1, and SR786 represent ALCL cell lines with the t(2;5) translocation. The non-t(2;5) T-cell lines are Molt4, CEM, and Jurkat. Tubulin is used as loading control. (C) Western blot analysis in B-cell lymphoid cell lines including 2 mantle cell lymphoma lines (Granta 519 and NCEB-1), 2 multiple myeloma cell lines (KMS12 and OPM2), and 2 follicular lymphoma cell lines (SUDHL4 and SUDHL10). The ALCL cell line Karpas 299 and the 2 carcinoma cell lines MCF7 and HeLa are used as controls. Tubulin is used as loading control. (D) Western blot analysis in primary T-cell lymphoma cases including 1 normal lymph node (LN), 4 primary ALK-positive (ALCL) lymphomas, 1 acute lymphoblastic leukemia (ALL), 2 adult T-cell leukemia/lymphoma (ATLL), and unspecified PTCL. Tubulin is used as loading control.