Expression of kinin receptors in response to PBMC exudates and kinins and chromatographic analysis of the hydrolysis of BK on rabbit smooth muscle cells. Rabbit smooth muscle cells were treated for 6 hours with BK, desArg⁹BK, IL-1β, 1% (vol/vol) exudates from monocytes stimulated with 1% (vol/vol) supernatant from an overnight culture of S aureus, or culture medium alone (control) in the absence of serum (A). After a washing step, cells were assayed for specific [³H]desArg¹⁰kallidin (B1R ligand; open bars) and [³H]BK (B2R ligand; filled bars) binding as described in “Materials and methods.” Specific binding is expressed as percent of control, where 100% is the binding to nontreated cells. The results represent mean ± SEM of at least 3 independent experiments done in triplicate. *P < .05 and **P < .01 compared with control values as determined by Student t test. NS indicates not significantly different from control values. Isotopically pure [³H]BK was incubated with rabbit smooth muscle cells. Supernatants were recovered after 3 hours (B), 6 hours (C), and 24 hours (D) and analyzed by HPLC as described in “Materials and methods.” Results are expressed as percent of total, where total is the total amount of eluted radioactivity. The result is representative of 3 experiments.