B1R and B2R mRNA expression in IMR-90 cells. IMR-90 cells were treated with 10 μM BK, 10 μM desArg⁹BK, 500 pg/mL IL-1β, 1% (vol/vol) PBMC exudates (supernatants from monocytes that had been stimulated for 24 hours with 1% S aureus overnight culture supernatants), 1% PBMC exudates in the presence of 10 μM BK, 1% PBMC exudates in the presence of 10 μM desArg⁹BK, or media alone in the absence of serum. Incubation times were 2 hours (A,C) and 6 hours (B,D). B1R (A-B) and B2R (C-D) mRNA expression was measured using quantitative real-time PCR and normalized to GAPDH mRNA levels. B1R and B2R mRNA expression in response to treatment with IL-1β was set to 100%. The figure presents the mean ± SEM of 3 independent experiments each performed in triplicate.