![Figure 5. Systemic administration of G-CSF promotes leukocyte trafficking in vivo and this response depends on G-CSFR expression by leukocytes. (A) Leukocyte trafficking in postcapillary venules of cremaster muscle in WT C57BL/6 mice 4 hours after intraperitoneal injection with saline or 10 μg G-CSF. n = 6 mice per group. *P < .05. (B) Leukocyte trafficking in postcapillary venules of cremaster muscle in WT mice reconstituted with WT bone marrow cells ([WT] → [WT]) 4 hours after intraperitoneal injection with saline or 10 μg G-CSF. n = 6 mice per group. *P < .01. (C) Leukocyte trafficking in postcapillary venules of cremaster muscle in bone marrow chimeric mice 4 hours after intraperitoneal injection with 10 μg G-CSF showing normal responses in G-CSFR−/− mice reconstituted with WT bone marrow cells ([WT] → [G-CSFR−/−]), but impaired G-CSF–induced leukocyte trafficking in WT mice reconstituted with G-CSFR−/− bone marrow cells ([G-CSFR−/−] → [WT]). n = 6 mice per group. *P < .05; **P < .005. Shown in panels A to C from left to right; leukocyte rolling flux, adhesion, and transendothelial migration. Data represent mean plus or minus SEM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/112/13/10.1182_blood-2008-02-139535/4/zh80240828160005.jpeg?Expires=1723422208&Signature=dBx0gZXsMq9vYV~CWVjEt2ZX8jfQ6e5asJpu~vYalBnm9ccC6oMJU4rRTwinAZM~g6Qoy6iSzRw42O8OtXTpR7oKXjD0ZdKlBqKYB01iSCWF8Z6uqFa1kmLXC3Z7N35DfgM3Ds1VQxEj1YvQ7QnP9eGs5WEHyqXODBBNm-pVoZa36C1~kHUYRbHNdNNZbyNg2QsVo5AASS8vW8OVlx-6zB9weN6vrGZ-cg0owQ9n2FuuEeab3U3KdbtaT2y1lMu50KSJ~27LUey4OUu4iMMi4E4NaW4N3QY0i0LF6zD~e1WwgkuBmYKh-Qiz1voBd7--VfAk5s0-tO3HNk1Ecp1~Iw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Systemic administration of G-CSF promotes leukocyte trafficking in vivo and this response depends on G-CSFR expression by leukocytes. (A) Leukocyte trafficking in postcapillary venules of cremaster muscle in WT C57BL/6 mice 4 hours after intraperitoneal injection with saline or 10 μg G-CSF. n = 6 mice per group. *P < .05. (B) Leukocyte trafficking in postcapillary venules of cremaster muscle in WT mice reconstituted with WT bone marrow cells ([WT] → [WT]) 4 hours after intraperitoneal injection with saline or 10 μg G-CSF. n = 6 mice per group. *P < .01. (C) Leukocyte trafficking in postcapillary venules of cremaster muscle in bone marrow chimeric mice 4 hours after intraperitoneal injection with 10 μg G-CSF showing normal responses in G-CSFR−/− mice reconstituted with WT bone marrow cells ([WT] → [G-CSFR−/−]), but impaired G-CSF–induced leukocyte trafficking in WT mice reconstituted with G-CSFR−/− bone marrow cells ([G-CSFR−/−] → [WT]). n = 6 mice per group. *P < .05; **P < .005. Shown in panels A to C from left to right; leukocyte rolling flux, adhesion, and transendothelial migration. Data represent mean plus or minus SEM.
