Body and spleen weight of aged Aire-deficient mice. (A) Body and (B) spleen weight were measured in 15-month-old Aire wild-type (WT) or knock-out (KO) mice kept in a conventional animal facility. Each symbol corresponds to a different animal. P values were calculated with t test for panel A and Mann-Whitney test for panel B. Mean values (A) or median (B) are indicated by horizontal lines. n = 4 WT males, 5 KO males (body and spleen weight); n = 5 WT females, 5 KO females (body weight); n = 13 WT females, 14 KO females (spleen weight).