Figure 2.
Figure 2. Differentiation and proliferation of erythroid progenitors. (A) Flow cytometry analysis of CD71 and TER119 expression of freshly harvested hematopoietic cells, with regions of maturing erythroid cells indicated as described.20 Table S1 presents the quantification. (B) CD71 and TER119 expression of E14.5 fetal liver cells after in vitro induction of KrasG12D for 4 days (day 0), then after 1 day of EPO stimulation (day +1), or after 2 days of EPO stimulation and 1 day of EPO withdrawal (day +3). (C) BrdU incorporation in vivo by CD71+TER119+ bone marrow cells. Note that the CD71/TER119 staining profile reflects lysis of erythrocytes and fixation required for BrdU staining. Mean percentage BrdU+ cells ± SEM is shown.

Differentiation and proliferation of erythroid progenitors. (A) Flow cytometry analysis of CD71 and TER119 expression of freshly harvested hematopoietic cells, with regions of maturing erythroid cells indicated as described.20  Table S1 presents the quantification. (B) CD71 and TER119 expression of E14.5 fetal liver cells after in vitro induction of KrasG12D for 4 days (day 0), then after 1 day of EPO stimulation (day +1), or after 2 days of EPO stimulation and 1 day of EPO withdrawal (day +3). (C) BrdU incorporation in vivo by CD71+TER119+ bone marrow cells. Note that the CD71/TER119 staining profile reflects lysis of erythrocytes and fixation required for BrdU staining. Mean percentage BrdU+ cells ± SEM is shown.

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