Figure 5
Figure 5. CCL2 mediates an anti-inflammatory role in vivo. (A) Asthma model: control (PBS-treated), OVA-primed mice (OVA), and OVA-primed mice injected intraperitoneally with 60 ng of CCL2 (CCL2) as described in “Methods” were analyzed for airway responsiveness on day 19. Values shown represent Δ Penn, which was calculated by subtracting control Penn measurements before antigen challenge from the Penn measurements after late or early antigen challenge. Baseline Penn levels were comparable among PBS-treated control, OVA-primed mice, and OVA-primed mice treated with CCL2. The results represent an average of 9 animals per treatment. (Bi-iii) Lung histology in mice that received CCL2 treatment. Histologic features of representative control (PBS), OVA-primed (OVA), and OVA-primed treated with low-dose CCL2 (CCL2) animals are shown. (Biv-vi) Immunohistochemical staining of the T cells in lung sections. (C) Bronchoalveolar lavage cell recovery in mice after treatment with CCL2. Percentage eosinophil recovery in BAL fluids in the various mice. Values represent the mean of 5 animals. (D) The peribronchial and perivascular inflammatory infiltrates were given an inflammatory score between 1 and 4 by a pathologist. The graph represents the average scores of 9 animals from each treatment group. (E,F) Arthritis model: AA was induced as described in “Rodent model for rheumatoid arthritis induction and assessment,” and rats immediately divided into 2 groups that were injected with low-dose CCL2 (240 ng in 300 μL PBS) or PBS. (E) A disease score between 0 and 4 was assigned to each limb, based on the degree of joint inflammation, redness, and deformity; the maximum possible score for an individual animal was 16. The graph represents the scores of 9 animals in each group that were measured every day. (F) Joint histology in PBS- or CCL2-treated mice. Results are presented as the mean plus or minus SEM of the difference between the 2 values for all the animals in each group. All photomicrograph images (B,F) were obtained with an Olympus AK 70 microscope (Center Valley, PA), Olympus Ach 20×/0.4 ∞/0.17. Camera make and model: Nikon Digital Camera DXM 1200F. Name and version number of image-acquisition software: Nikon ACT-l for DMX1200F.

CCL2 mediates an anti-inflammatory role in vivo. (A) Asthma model: control (PBS-treated), OVA-primed mice (OVA), and OVA-primed mice injected intraperitoneally with 60 ng of CCL2 (CCL2) as described in “Methods” were analyzed for airway responsiveness on day 19. Values shown represent Δ Penn, which was calculated by subtracting control Penn measurements before antigen challenge from the Penn measurements after late or early antigen challenge. Baseline Penn levels were comparable among PBS-treated control, OVA-primed mice, and OVA-primed mice treated with CCL2. The results represent an average of 9 animals per treatment. (Bi-iii) Lung histology in mice that received CCL2 treatment. Histologic features of representative control (PBS), OVA-primed (OVA), and OVA-primed treated with low-dose CCL2 (CCL2) animals are shown. (Biv-vi) Immunohistochemical staining of the T cells in lung sections. (C) Bronchoalveolar lavage cell recovery in mice after treatment with CCL2. Percentage eosinophil recovery in BAL fluids in the various mice. Values represent the mean of 5 animals. (D) The peribronchial and perivascular inflammatory infiltrates were given an inflammatory score between 1 and 4 by a pathologist. The graph represents the average scores of 9 animals from each treatment group. (E,F) Arthritis model: AA was induced as described in “Rodent model for rheumatoid arthritis induction and assessment,” and rats immediately divided into 2 groups that were injected with low-dose CCL2 (240 ng in 300 μL PBS) or PBS. (E) A disease score between 0 and 4 was assigned to each limb, based on the degree of joint inflammation, redness, and deformity; the maximum possible score for an individual animal was 16. The graph represents the scores of 9 animals in each group that were measured every day. (F) Joint histology in PBS- or CCL2-treated mice. Results are presented as the mean plus or minus SEM of the difference between the 2 values for all the animals in each group. All photomicrograph images (B,F) were obtained with an Olympus AK 70 microscope (Center Valley, PA), Olympus Ach 20×/0.4 ∞/0.17. Camera make and model: Nikon Digital Camera DXM 1200F. Name and version number of image-acquisition software: Nikon ACT-l for DMX1200F.

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