Figure 1
![Figure 1. DNA synthesis and mRNA levels in HUVECs. HUVECs were treated with 50 μM DL-Hcy and 1 mM AZC in control medium for 48 hours, or 300 nM TSA and 5 mM NaBr for 24 hours. (A) Thymidine uptake. Cells were metabolically labeled with 1 μCi (0.037 MBq)/mL [3H]-thymidine during the last 3 hours. Values represent mean (± SD) from 3 separate experiments with triplicates (n = 9). *P < .01 versus non-Hcy and nonviral control. #P < .01 versus Hcy-treated and non–viral-transduced group. (B) Northern blot analysis. Total cellular RNA was used for Northern analysis (10 μg). The blot was hybridized with the probes of human cyclins A and D1, and subsequently with 18S RNA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/10/10.1182_blood-2007-06-096701/6/zh80230709760001.jpeg?Expires=1724234140&Signature=LL8pb5fcm-17WAOMYL6AjjCJZmBLemFcxtb~4KIIUZn3DscAHLXnFqTnxGndt1604TYzyNQ~fJrn13OobdAVxUe7s4p1Wu25jftE6ebDFBfYTsOxCaP8o0i0bYWQ~~Ri68F6jt6XOOZ7CJmQQbGP87Rgbsz0aqnXWVM4aPJ4-D5imGNIjKbHiFECaQoIFAFQsTVTl5sBzSWoYDzlzg8qVQnDD6vm~1qJUBFt3zgBc6aAx0sHQpaOsoDud9SNvt1I5m9pKzTlH4eCZdfaWNypDxeS6TBy7zHFlmo39PBTs3MS-s1oAEK1jy-nh3sM~73Wn2xGf17TosXtszJRe9oaBQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DNA synthesis and mRNA levels in HUVECs. HUVECs were treated with 50 μM DL-Hcy and 1 mM AZC in control medium for 48 hours, or 300 nM TSA and 5 mM NaBr for 24 hours. (A) Thymidine uptake. Cells were metabolically labeled with 1 μCi (0.037 MBq)/mL [3H]-thymidine during the last 3 hours. Values represent mean (± SD) from 3 separate experiments with triplicates (n = 9). *P < .01 versus non-Hcy and nonviral control. #P < .01 versus Hcy-treated and non–viral-transduced group. (B) Northern blot analysis. Total cellular RNA was used for Northern analysis (10 μg). The blot was hybridized with the probes of human cyclins A and D1, and subsequently with 18S RNA.
