Partial reversion of the osteopetrotic phenotype in DC-treated oc/oc mice. (A) Distribution of the age of death of control oc/oc (n = 26), DC-treated oc/oc mice from 3 independent experiments (n = 14) and oc/oc treated with DCs purified from CD11c-DTR mice and diphtheria toxin (DT) (n = 4). (B) Radiographic analysis of normal mice, PBS-treated oc/oc mice, DC-treated oc/oc mice that survived more than 33 days (n = 6), and oc/oc mice treated with DCs from CD11c-DTR mice and DT. Arrows indicate the medullar cavity. (C) Histologic analysis of the tibia of normal littermates, control, and DC-treated oc/oc mice after van Gieson and von Kossa staining. GP indicates growth plate. The results are representative of the mean phenotype observed in 3 independent experiments. Images were captured using a DMLB microscope (Leica, Wetzlar, Germany), a 3CCD color video DXC-390 camera (Sony, Heerlen, The Netherlands) and the Osteomeasure Analysis System (Osteometrics, Atlanta, GA). (D) Bone morphometric analysis on +/+ mice (n = 3), PBS-treated oc/oc mice (n = 3), and DC-treated oc/oc mice (n = 6). The results are the distribution of the values measured for each animal. MdV/TV indicates mineralized volume/tissue volume. Correspondence with the radiographic analysis (C): ★ represents mouse 1; ■, mouse 2; ○, mouse 3; ♦, mouse 4; X, mouse 5; and ▲, mouse 6. (E) Statistical analysis of parameters from panel D. Parameters from control oc/oc mice were significantly different from those of DC-treated oc/oc mice group 1 (mice nos. 1-3) presenting a small bone marrow cavity (P < .01). A second group of DC-treated oc/oc mice, group 2 (mice nos. 4-6), presented a larger bone marrow cavity, and their histomorphometric parameters were not significantly different from normal +/+ mice (P > .05).