Histopathologic examination of KMS11-derived tumors treated with AdCMV.NK4. (A) Hematoxylin and eosin staining of AdCMV.lacZ-treated, AdCMV.NK4-treated, Dex-treated, and AdCMV.NK4 plus Dex-treated KMS11 tumors. The arrows indicate areas of tumor cell death. (B) TUNEL assay of KMS11 tumors. The numbers of TUNEL+ cells per one visual field (0.148 mm2) were counted and shown in the form of a bar graph. * P = .001, ** P = .008 compared with AdCMV.lacZ-treated tumors. (C) Immunohistochemical analysis of blood vessels in subcutaneous tumors derived from KMS11 cells. Vascular endothelial cells were stained with anti-VWF antibody. The mean number of blood vessels per one visual field is indicated by the bar graph. *P = .029 compared with that in AdCMV.lacZ-treated tumors. (D) Immunohistochemical staining of activated c-Met in AdCMV.NK4-treated tumors using anti–phosphorylated c-MET. To eliminate background staining, human IgG was used as a control. In panels B and C the mean ± 1 SD (n-7 for B and n-5 for C) is shown.
