Figure 2
Figure 2. Competition of SSL5 and mAbs directed against surface-expressed receptors in binding to neutrophils, monocytes, and lymphocytes. Neutrophils (▪), monocytes (⊡), and lymphocytes (□) were incubated with 10 μg/mL SSL5 for 15 minutes on ice. The cells were then stained with a panel of FITC-, PE-, or APC-conjugated mAbs directed against cell-surface–expressed receptors in the presence of 10% heat-inactivated serum. Data depict fold increase or decrease of antibody binding to SSL5-treated cells compared with control-treated cells. The data are mean values of 3 independent experiments.

Competition of SSL5 and mAbs directed against surface-expressed receptors in binding to neutrophils, monocytes, and lymphocytes. Neutrophils (▪), monocytes (⊡), and lymphocytes (□) were incubated with 10 μg/mL SSL5 for 15 minutes on ice. The cells were then stained with a panel of FITC-, PE-, or APC-conjugated mAbs directed against cell-surface–expressed receptors in the presence of 10% heat-inactivated serum. Data depict fold increase or decrease of antibody binding to SSL5-treated cells compared with control-treated cells. The data are mean values of 3 independent experiments.

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