Effects of MCL-1 antisense oligonucleotides on MCL-1 protein expression and cell viability in HMC-1.1 and HMC-1.2 cells. HMC-1.1 cells (A-C) and HMC-1.2 cells (D-F) harboring KIT D816V were transfected with an MCL-1 antisense oligonucleotide at 250 nM (Antisense), a scramble control, or were left untransfected (Control) for 12 hours before analysis. (A, D) Western blot analysis of MCL-1 expression was performed using an anti–MCL-1 antibody. β-Actin served as loading control. One representative blot is shown for each cell line. (B, E) Evaluation of nonviable (trypan blue-positive) expressed as a percentage of all nucleated cells. (C, F) Numbers (%) of apoptotic cells. Results represent the mean ± SD from 3 independent experiments.