Prediction of HLA-binding peptides encoded by the FLT3-ITD sequence identified in AML cells of patient J.C. (JC-FLT3-ITD). Peptides from the ITD protein region (boxed) were predicted with public domain algorithms^13-16  combining predictions for proteasomal processing, transporter associated with antigen processing (TAP) transport, and binding to the patient's HLA-A and -B alleles. Five peptides, ranked either first or second by the algorithms, were chosen for the in vitro stimulation of JC-PBMCs. The amino acid sequences of these peptides, their respective presenting HLA-I alleles, and their prediction scores and ranks are indicated (n.a. indicates prediction not available for this allele/epitope length).