Migration and motility in response to SDF-1. (A) Increased motility of plasma cells in response to SDF-1 with pseudopodia formation. (B) Transwell migration assay demonstrating the migration of MM.1S and U266 MM cell lines in response to serial concentrations of SDF-1 (0-100 nM). SDF-1 induced maximum migration at doses of 20 to 30 nM and decreased migration at doses of 75 to 100 nM. (C) Transwell migration assay demonstrating the migration of CD138⁺ primary plasma cells in response to SDF-1.