Inhibiting Cox2 activity attenuates BMP6-induced MEC proliferation, migration, and tube formation. (A) Time-dependent response of MEC proliferation to BMP6 (100 ng/mL) treatment. MECs were pretreated with NS398 (10 μM), SC560 (100 nM), or vehicle before treatment with BMP6. Cells were counted at each time point. (B) MEC cell migration on gelatincoated filters to BMP6 was assayed after pretreatment with NS398, SC560, or vehicle. After 6 hours, transmigrated cells were counted. (C) MECs were plated on Matrigel-coated 24-well plates, followed by treatment with BMP6, NS398, SC560, and/or vehicle. Images were taken 6 hours after incubation. Original magnification, × 10. A Nikon 10×/0.25 NA objective lens (Nikon, Melville, NY) was used along with QCapture 2.6.0 software (Quantitative Imaging, Burnaby, BC, Canada). (D) The mean and SD of 3 replicate wells of a representative experiment is shown. The p-value was determined by Student t test.