RT-PCR and Western blotting analysis of gene expression in BMP6-induced EC activation. (A) RT-PCR analysis of Wnt2, Cox2, Timp1 and Adamts1 expression using total RNA from MECs collected at 0, 4, 12, and 24 hours after BMP6 treatment. GAPDH was used as a loading control. (B) Western blot analysis of Cox2 protein expression after BMP6 stimulation for the indicated times. β-actin was used as a loading control.