Characterization of the hematopoietic progenitors repopulating the BM of FA-D1 recipients after transplantation with EGFP- or hBRCA2-transduced FA-D1 cells. (A) Quantification of the proportion of CFCs present in the BM of FA-D1 recipients of donor origin (top panel) harboring BRCA2-LV or EGFP-LV proviruses (bottom panel). qPCR analyses of SRY sequences and of BRCA2 and EGFP-LV proviruses were determined in individually picked colonies generated by BM samples obtained 2 and 6 months after transplantation. (B) Kinetics of hBRCA2 mRNA expression in BM samples from 3 FA-D1 recipients transplanted with BRCA2-transduced cells. BM samples were periodically aspirated from the femora of recipient mice and analyzed by RT-PCR. Data show the increase in hBRCA2 expression compared with a mouse transplanted with EGFP-transduced cells (mouse 9).