LPS stimulation increases the association of Bim with A1 and Bcl-x_L in B cells. (A) Western blots for Bcl-x_L and A1 from wt and nfkb1^−/− B cells stimulated with LPS for the indicated times. The data are representative of 3 experiments. (B) Bim immune precipitates from lysates of untreated or LPS-stimulated (2, 6, and 12 hours) wt and nfkb1^−/− B cells analyzed by Western blotting for Bcl-2, A1, and Bcl-x_L expression. (C) Spontaneous and LPS-induced apoptosis in B cells from wt, c-rel^−/−, bim^−/−, or c-rel^−/−bim^−/− mice. The data represent the mean plus or minus SEM from 4 experiments. (D) Spontaneous and LPS-induced apoptosis of B cells from wt, nfkb1^−/−, c-rel^−/−, or nfkb1^−/−c-rel^−/− (all with or without the bcl-2^T). The data represent the mean plus or minus SEM from 3 experiments.