Transgenic Bcl-2 protects nfkb1^−/− B cells from spontaneous and LPS-induced apoptosis. Purified B cells from wt, nfkb1^−/−, bcl-2^T, and nfkb1^−/−bcl-2^T mice were cultured with or without LPS for the indicated times. (A) Levels of cell death. The data are mean plus or minus SEM for 3 experiments. (B) B-cell proliferation. Results represent mean plus or minus SEM of 3 experiments, each performed in triplicate. (C) Bcl-2, Mcl-1, Bcl-x_L, and A1 expression in B cells after LPS stimulation. This blot is representative of 3 experiments.