Comparison of the capacity to produce IFN-α and IFN-γ by subsets of pDC preparations. The pDC preparation and the subsets considered as pDCs (CD49b⁻) or as IKDCs (CD49b⁺) were isolated as in Figure 2. The sorted cells (10⁵) were cultured in 0.2 mL medium for 48 hours (IFN-α) or for 65 hours (IFN-γ). The stimulus used was CpG2216 for IFN-α production, IL-12 and IL-18 for IFN-γ production. Results are the means plus or minus SD of 5 individual cultures, from one experiment typical of 3.