Figure 6
Cell-to-cell contact dependence required for PDC activation of IFN-γ–producing CD3+ T cells. Immunomagnetic bead-isolated PDCs were separated from CD3+ T cells using a Transwell filter with membrane pore size of 0.4 μm. Cell contact between PDCs and CD3+ T cells in the presence of EBV generated significantly greater number of IFN-γ–producing CD3+ T cells compared to the soluble factors secreted by PDCs in response to EBV (155.0 ± 7.1 compared to 27.0 ± 2.8 spots/3 × 105 T cells, Student t test P < .01). Results are expressed as mean ± SEM.
