Confirmation of GC-regulated gene expression in monocytes by flow cytometry. Expression of selected genes found to be differentially expressed by microarray analysis was confirmed by flow cytometry. Monocytes were treated with 10 nM fluticasone for 16 hours or left untreated and were tested afterward for expression of the cell-surface molecules CD163, CD11a, and CD36 or for intracellular expression of sin3A-associated protein (SAP30). Specific profiles are shown by thick lines and isotype controls appear as spotted lines. Numbers show the quotient of specific/isotype control mean fluorescence intensity (MFI). The experiment was done 4 times with similar results, and the differences in MFI shifts between control and GC-treated cells were statistically significant for every protein analyzed (P < .05, Student t test, for CD11a, CD36, and SAP; P < .001 for CD163).