Cytokine profile of LNCs after Th1 and Th2 exposures. M tuberculosis–sensitized C57BL/6 (A) and Ascaris-sensitized BALB/c (B) mice were treated with vehicle, prednisolone, or STA-5326. Results are representative of 4 Th1 and 6 Th2 in vivo studies. In the studies, to calculate deviation, LNCs from sensitized mice were individually harvested and tested in triplicate from each mouse for cytokine production in response to immobilized anti-CD3ϵ antibody and soluble anti-CD28 antibody. Lymph nodes from naive mice in the Th1 study were pooled and tested for cytokine production in triplicate. IFN-γ (A) in the Th1 study and IL-4 (B) in the Th2 study were analyzed with supernatants collected at 24 hours and 48 hours, respectively. Each dot represents an individual mouse, and the bar drawn in each group represents the mean value. **P < .01 compared with the level of the vehicle group.