Higher in vitro differentiation capacity of SSEA-1⁺. Comparative studies of in vitro differentiation capacity (n = 3-5) of SSEA-1⁻ and enriched SSEA-1⁺ populations (∼60%-70% purity). FACS analysis shows the level of specific antigen expression for each lineage tested: osteogenic (osteocalcin and bone sialoprotein II [BSP II]), myogenic (dystrophin and desmin), astrocyte (GFAP), hepatocyte (albumin and HNF-1), and endothelial (extracellular CD31 and VWF). Gray and white areas are the isotype control and specific antibody staining, respectively. The numbers shown indicate the levels of expression for each antigen that were calculated using the mean fluorescence intensity (MFI) values obtained from each specific staining over the values from their respective isotype controls. The specificity of the antibodies used in this study was first confirmed by Western-blot analysis (data not shown).