Structure and trafficking to Maurer clefts. (A) Localization of the resident Maurer clefts markers PfSBP1 and PfMAHRP1 in parental CS2 (first row of each panel) and CS2ΔSBP1-infected cells (second row of each panel). PfMAHRP1 was correctly trafficked to Maurer clefts in both cell lines, whereas PfSBP1 showed only cross-reactivity with another protein within the parasite CS2ΔSBP1 without localization on Maurer clefts as observed in the parental CS2 cells. (B) Ultrastructural analysis of CS2- (top) and CS2ΔSBP1-infected cells (bottom) shows typical Maurer clefts in both cell lines. Bars represent 0.5 μm.