Profile of PKC isoform expression in mature B lymphocytes. (A) Quantitation of PKCβII expression in CLL cells by Western blotting. Equal amounts of protein were used from each CLL patient, and the intensity of the PKCβII-reactive band was compared with that generated from known amounts of recombinant PKCβII. β-Actin was used to indicate equal protein loading. (B) Western blot analysis of PKC isoform expression by CLL, HCL, and CD19-purified normal B cells. Equal amounts of protein were analyzed for each PKC isoform and cell type. This figure is representative of 3 separate experiments with different patients. (C) Western blot analysis of PKCβII expression in CLL cells, mantle cell lymphoma (MCL) cells, marginal zone lymphoma (MZL) cells, follicular lymphoma (FC) cells, and plasma cell leukemia (PCL) cells. Equal amounts of protein were loaded for each cell type. (D) Flow cytometric analysis of PKCβII expression in permeabilized CLL and HCL cells. This figure is representative of 2 separate experiments in different patients with CLL and HCL.