Activation status of the WNT pathway in MCL. (A) Western blot studies revealed the strong expression of p-GSK3β in all 3 MCL cell lines. In addition, DvL-2 was highly expressed. Importantly, the slowly migrating/phosphorylated forms of DvL-2 were detected in all 3 cell lines. Mouse stem cell lysates were used as positive controls for the phosphorylated forms of DvL proteins. P indicates the phosphorylated form of DvL-2 and UnP indicates the unphosphorylated form of DvL-2. (B) Western blot studies revealed the expression pattern of p-GSK3β in 5 MCL primary tumors. P1, P3, and P5 had a relatively high level of p-GSK3β, which was associated with relatively a high level of DvL-2 and the presence of its slowly migrating forms. In contrast, P2 and P3 had weak or undetectable p-GSK3β, which correlated with a weak DvL-2 expression.