Comparison of cell-cycle entry and activation of adult T cells following exposure to HΔ24/FΔ30-LVs or VSV-G-LVs. Resting adult CD3⁺ T cells were transduced with HΔ24/FΔ30- or VSV-G–displaying LVs, or no vector, and as a control IL-7–prestimulated T cells were transduced with HΔ24/FΔ30-displaying LVs. (A) Cell-cycle progression was monitored by simultaneously visualizing the RNA (pyronin-Y) and DNA (7-AAD) content of the T cells at day 3 of incubation. The percentages of cells in the G0/G1a, G1b, and S/G2/M phase of the cell cycle are indicated in the dot blots. (B) Surface staining for CD69, HLA-DR, and CD71 versus GFP⁺ cell expression is shown at day 3 after transduction. The percentage of activation marker–expressing GFP⁺ cells is indicated in the top right quadrant; the percentage of GFP⁺ cells not expressing the activation marker is indicated in the bottom right quadrant.