Effects of LAQ824 on the expression of LPS-induced proinflammatory genes in human monocyte-derived macrophages and DCs. (A) mRNA levels of proinflammatory genes, lipid mediators, and chemotactic factors in macrophages treated with indicated stimuli. Color bars represent the ratio of hybridization signals between corresponding time points and time 0, according to the scale shown on the left. (B) Protein secretion of selected proinflammatory genes from macrophages and DCs. Expression levels are determined by Cytometric Bead Array chemokine kit (BD Pharmingen). Representative of 4 independent experiments. (C) CCR5 surface expression in DCs incubated with indicated stimuli. Expression level is determined by flow cytometry.