Apoptosis in HeLa cells transfected with rps19 siRNAs. (A) DAPI staining of cells detaching from the culture dish 48 hours after transfection with siRNA rps19-1 shows hypercondensed chromatin, similar to treatment with 10 μM cis-platin (CDDP) for 24 hours. (B) Immunodetection of the 85 kDa fragment of cleaved PARP on Western blot. “Ctrl” indicates untransfected cells. (C) Cleaved PARP is detected by immunofluorescence in RPS19-depleted cells but not in cells transfected with scramble or lamin A/C siRNAs.