Dose-dependent induction of IL-8 secretion by heat-inactivated bacteria in HEK 293 cells stably transfected with human TLR2 or human TLR4/MD-2. TLR2 is shown in panel A or human TLR4/MD-2 in panel B. Duplicates were performed for all conditions tested. This represents 1 of 2 representative experiments. Error bars show ± SEM.