Flow cytometry quantification of the expression of CD14, TLR2, TLR4, and MD-2 on human monocytes, polymorphonuclear neutrophils, human umbilical vein endothelial cells (HUVECs), and human bronchial BEAS 2B cells. Antibodies (5 μg/mL) were incubated with cells, and binding was revealed with an APC-conjugated goat anti–mouse IgG secondary antibody.