Slp2a recruitment on vesicular structures requires Rab27a. (A) Confocal microscopy of wild-type LAK-T cells transfected with GFP-Slp2a-SHD and DsRed-Rab27a. (B) Confocal microscopy of wild-type LAK-T cells transfected with GFP-Slp2a-SHDmut and Dsred-Rab27a. (C) Confocal microscopy of wild-type LAK-T cells transfected with GFP-Slp2a-C2 and DsRed-Rab27a. (D) Confocal microscopy of GS2 LAK-T cells transfected with GFP-Slp2a-C2 and labeled with an antiperforin (red). Scale bars represent 5 μm. Data are representative of at least 3 independent experiments.