Tissue expression of Slp2a isoforms. (A) A schematic representation of the exon combinations (exons 10 to 14) that define 2 splicing isoforms of Slp2a (KIAA1597 and Slp2a-hem). The location of 3 putative PEST-like sequences (▽) and the phosphatase-sensitive region of Slp2a-hem leading to the cleaved form (▲) are shown. The locations of the PCR primers used to specifically amplify each Slp2a isoform are indicated by arrows. (B) RT-PCR analysis, using commercial cDNA panels from nonhematopoietic and hematopoietic tissues, and cDNA from purified human NK cells. The couples of primers shown in panel A were used to amplify each Slp2a isoforms in hematopoietic and nonhematopoietic cDNA panels. Actin expression was used for normalization.