Pam3Cys and LPS induce NF-κB nuclear translocation in MSCs. MSCs were plated in MSC medium. When the cells reached confluence, 10 μg/mL Pam3Cys or LPS was added in 10% FCS containing DMEM. MSCs were harvested at 15 minutes, 1 hour, or 4 hours, and nuclear and cytpalsmic proteins were extracted. The nuclear (A,C) and cytoplasmic (B,D) extracts were quantified, run on SDS-PAGE gel, and blotted with anti–NF-κB p65, antinucleolin, or anti–total ERK antibodies. The autoradiographs were quantified by densitometry (C-D).