Normal macrophage survival and cytokine production on TLR stimulation. (A) Apoptosis rate of Mcl-1–deficient macrophages with or without PAMP stimulation. Peritoneal macrophages from Mcl-1–deficient (KO) and control mice (ctrl) were stimulated with the indicated PAMPs for 16 hours and examined for apoptosis using the LIVE/DEAD Viability/Cytotoxicity kit. (B) Inflammatory cytokine production by Mcl-1–deficient macrophages after PAMP stimulation. Peritoneal macrophages from Mcl-1–deficient (▪)and control (□)mice were stimulated with the indicated PAMPs overnight and measured for cytokine productions by ELISA. Shown are mean and SD of triplicate determinations. Data are representative of 3 experiments.