Effect of HoxD3 on endothelial-cell migration and integrin expression. (A-B) HUVECs were transfected with scrambled or MLL siRNA oligonucleotides and empty vector or HoxD3 wild-type plasmid. (A) HUVEC migration was analyzed using the scratched wound assay. Data are shown as percent scrambled + mock and mean ± SEM. *P < .05 versus mock + scrambled siRNA, #P < .05 versus mock + MLL siRNA, n = 3. (B) RT-PCR analysis of MLL, HoxD3, and integrin mRNA expression. GAPDH serves as loading control.