HoxA9 specifically rescues endothelial-cell migration and EphB4 expression. (A) HUVECs were transfected with siRNA against MLL or scrambled (scr) oligonucleotides and empty vector or HoxA9 wild-type plasmid. HUVEC migration was analyzed using the scratched wound assay. Data are shown as percent scrambled + mock and mean ± SEM. *P < .05 versus mock + MLL siRNA, n = 4. (B) RT-PCR analysis of EphB4 mRNA expression following MLL siRNA or scrambled oligonucleotide transfection. A representative gel is shown. (C) Expression is given as percent scrambled. Data are mean ± SEM. *P < .05, n = 3. (D-F) HUVECs were transfected with scrambled or MLL siRNA oligonucleotides and empty vector or HoxA9 wild-type plasmid. (D) Western blot analysis was performed to investigate EphB4 protein expression. Tubulin is shown as loading control. (E) RT-PCR analysis of EphB4 mRNA expression. A representative gel is shown. GAPDH serves as loading control. (F) Semiquantitative analysis of EphB4 mRNA expression. Data are shown as percent scrambled + mock and mean ± SEM, *P < .05 versus mock + MLL siRNA, n = 6.