Histone H3 lysine K4 methylation is present at the HoxA9 and HoxD3 genes. (A-D) ChIP was performed in HUVEC lysates using an antibody directed against di-methylated histone H3 lysine K4. (A) Overview of primer pairs (P1-P3 for HoxA9 and P4-P6 for HoxD3) amplifying the indicated promoter or exon regions within the HoxA9 and HoxD3 genes. (B) Western blot analysis was performed to confirm the immunoprecipitation against di-methylated histone H3 lysine K4. (C-D) Representative PCR with primers detecting the indicated promoter or exon regions of the HoxA9 or HoxD3 genes are shown, n = 3. Ab indicates antibody control.