MLL regulates endothelial-cell migration and sprouting. (A,C) HUVECs were transfected with 2 different siRNAs against MLL (I and II) or scrambled oligonucleotides. (A) RT-PCR analysis of MLL mRNA expression. A representative gel is shown. GAPDH serves as loading control. (B,D) HUVECs were transfected with MLL siRNA or scrambled oligonucleotides. (B) Cell migration was measured using a scratched wound assay. Data are shown as mean ± SEM. *P < .05, n = 3. (C) A spheroid assay was performed to analyze basal endothelial sprouting capacity. Representative spheroids are shown. (D) Analysis of endothelial sprouting capacity with or without bFGF (30 ng/mL) stimulation. Endothelial sprouting capacity is given as cumulative sprout length per spheroid. Data are shown as mean ± SEM. *P < .01 versus scrambled and #P < .01 versus scrambled + bFGF, n = 3. (E) HUVECs were prestimulated with different doses of the methyltransferase inhibitor MTA as indicated and stimulation was repeated following scratch procedure. Migration was measured using the scratched wound assay. Data are shown as percent control and mean ± SEM. *P < .05 versus control, n = 3. (F) HUVEC capillary sprouting was analyzed in the presence of different doses of MTA as indicated. Data are shown as percent control and mean ± SEM. *P < .05 versus control; #P < .01 versus control, n = 3.