HNRPK transcription-regulatory activity is dispensable for BCR/ABL oncogenic potential. (A) Western and Northern blots show levels of Myc and/or HA-tagged wild-type and mutant C299-HNRPK in 32D-BCR/ABL cells transduced with the empty MigR1 vector (lane 1), wild-type HNRPK (lane 2), and C299-HNRPK dominant-negative mutant (lane 3). (B) Confocal microscopy of anti-HA-stained wild-type and C299-HNRPK-expressing 32D-BCR/ABL cells. (C) Effect of wild-type and C299-HNRPK on growth factor-independent clonogenic efficiency of 32D-BCR/ABL cells.