Cavtratin inhibits CCL2-stimulated migration of monocytic cells across BMECs. BMECs were cultured to confluence and then pretreated with 2 μm cavtratin added to both the top and bottom Transwell chambers. After 24 hours, monolayers were washed, fresh media containing WEHI cells were added to the top chamber, and CCL2 (10 nM) was applied to the lower chamber. At 18 hours after the application of chemokine, transmigrated WEHI cells were collected from the lower chamber and quantified as detailed in “Materials and methods.” Relative transmigration was calculated as x-fold increase over control (lacking both CCL2 and cavtratin). The data are presented as mean ± SE and represent 3 independent experiments performed in triplicate. #P < .01 when compared with control; *P < .01 when compared with 10 nM CCL2 treatment alone.